Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKPDISELSVEELKRLQEEAEALIASKKDQAIEDAYNQIIEIAENVGFSVEQLLEFGAQKRKKTTRKSVEPRYRNKNNAEETWTGRGKQPRWLVAEIEKGAKLEDFLI 5JBH Chain:L ((2-52)) -----------------QKARIKIASTNVRSLDEVANQIKQIAERTGVRMSGPIPLPTKRIRITTRKS----------------------------------------

General information: TITO was launched using: RESULT: Template: 5JBH.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain L - contact count / total energy / energy per contact / energy per residue : 52 -9061 -174.25 -177.67 target 2D structure prediction score : 0.45 Monomeric hydrophicity matching model chain L : 0.69 3D Compatibility (PKB) : -174.25 2D Compatibility (Sec. Struct. Predict.) : 0.45 1D Compatibility (Hydrophobicity) : 0.69 QMean score : 0.538

(partial model without unconserved sides chains): PDB file : Tito_5JBH.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5JBH-query.scw PDB file : Tito_Scwrl_5JBH.pdb: