Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MLKAQKLTLAVLISAAIISSAQASEQSEAKGFVEDANGSILFRTGYLTRDKKQGAKDTSSV--AQSAIVSIESGFTPGIVGFGVGVVGDGSFKIGENKNTGNQMIPKHNDGSAYDHWARGGGSVKARFSNTTVRYGTQVLDLPVLASNTGRMVPEYFTGTLLTSHEIKNLEVVAGKFTKDQMSDQINTD---------ADASGRGLDRAIVWGAKYKFNDNLNASYYGLDSKNALERHYANVNFKQPLANDSSLTYDFSGYHTKFDANAHTYSATGTVAPNYAADGIADEEKTNNIWAISGTYATGPHSVMLAYQQNTGNVGYDYGQNADGFQSIYLPN-SYMSDFIGNHEKSAQIQYNVDFGKLGVLPGLNWTTAFVYGWDIKVRNVTDDAQEREFFNQVKYTVQSGFAKDASLRIRNSYYRASDAYQGAYIGDTNEWRIFLDIPVKLF 4FSP Chain:A ((13-408)) -------------------------QGLEEGFLEDSRASLALRNFYMNRDFR------KSEEWAQGFLFDYRSGYTEGTLGVGLDLLGKLGVRLD---------------------YARLDATAKLRLSRSELKVGGLVPKLPTIQPNYGRLFPQVFQGALLTSGELSGLSLNLGRLTEVS-------SDLALFNRNRRFAGAAQADRFDLAGLDYRIAPDWTGSYHYGELEQVYAQHFLGLKGRIGIAAD-SLESDL-----RLALSRDTGGARGGRIDNRSFSGSLTYRLRNG------------QAFGLGYQRMSGDHGFPYLEGTD----PYLVNFGQYNDFAEAGESSWQLRYDCDFAPLGV-PGLSLMTRYFSGHGAKPKGA-DGSREWERDSDLRYVLQGGALKGLGLVWRNATYRS------AFSRDIDENRLYLTYELPLF

General information: TITO was launched using: RESULT: Template: 4FSP.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1964 65633 33.42 188.06 target 2D structure prediction score : 0.48 Monomeric hydrophicity matching model chain A : 0.73 3D Compatibility (PKB) : 33.42 2D Compatibility (Sec. Struct. Predict.) : 0.48 1D Compatibility (Hydrophobicity) : 0.73 QMean score : 0.317

(partial model without unconserved sides chains): PDB file : Tito_4FSP.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4FSP-query.scw PDB file : Tito_Scwrl_4FSP.pdb: