Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MAIRRIEGLLHLASEGRYAILVGRFNSFVVEHLLEGAIDTLKRHGVNEDNITVIHAPGAWELPIVAKKLATSNQFDAIIALGAVIRGSTPHFDFVAGECAKGLGVVALESSLPVINGVLTTDSIEQAIERSG--TKAGNKGSEAALTAIEMVNLLKAI 1W29 Chain:A ((15-139)) ------------ASGVRLAIVASSWHGKICDALLDGARKVAA--GCGLDDPTVVRVLGAIEIPVVAQELARNH--DAVVALGVVIRGQTPHFDYVCDAVTQGLTRVSLDSSTPIANGVLTTNTEEQALDRAGLPTSAEDKG-----------------

General information: TITO was launched using: RESULT: Template: 1W29.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 575 -96489 -167.81 -784.46 target 2D structure prediction score : 0.64 Monomeric hydrophicity matching model chain A : 0.77 3D Compatibility (PKB) : -167.81 2D Compatibility (Sec. Struct. Predict.) : 0.64 1D Compatibility (Hydrophobicity) : 0.77 QMean score : 0.581

(partial model without unconserved sides chains): PDB file : Tito_1W29.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1W29-query.scw PDB file : Tito_Scwrl_1W29.pdb: