Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTDTRENWTSRSGFIIAAVGSAVGLGNIWRFPYVAYENGGGAFLIPYLLALITAGLPLLFLDYAVG-HRSTGSPPKAYR--ALFKGGETLGWWQVCVCIIIGLYYASVLTWA---------GSYVYFSIGQMWGSDP--------EGF------FFNTY-LQTTKATGFDLQFVGHLFWPIVGIWALTLIILYGGVKKGVELSNKI--FMPLL-FILFTILVIQSLRLPGAVQGLNAFFTPNWSAMMDYKVWLAAYGHTFFSLSVGFGIMVTYASYLKPKTNLTGSGLIVGFANASTEILAGIGIFAALGFMAHAAGTEVKDVVSGGIGLAFIAFPKIISSLGSGADLFGLLFFSSLFVAGISSMVSILEVPIAAMQDKLKWGRKKAVTIIGGGSALVSIILFSSVNA--IKLVDIVDHFINNIGIIGGALLSIISVAWFKRSALKELRDHVNRISTIQLGKGWDFTLTVITSLILLTTLCMTVFNLIKNGYDTYS----MSLQGVFGW----GSVIFCAVVAIVLSKMKDR 4XP1 Chain:A ((1-511)) --DERETWSGKVDFLLSVIGFAVDLANVWRFPYLCYKNGGGAFLVPYGIMLAVGGIPLFYMELALGQHNRKGAITCWGRLVPLFKG---IGYAVVLIAFYVDFYYNVIIAWSLRFFFASFTNSLPWTSCNNIWNTPNCRPFEGHVEGFQSAASEYFNRYILELNRSEG--IHDLGAIKWDMALCLLIVYLICYFSLWKGISTSGKVVWFTALFPYAVLLILLIRGLTLPGSFLGIQYYLTPNFSAIYKAEVWVDAATQVFFSLGPGFGVLLAYASYNKYHNNVYKDALLTSFINSATSFIAGFVIFSVLGYMAHTLGVRIEDVATEGPGLVFVVYPAAIATM-PASTFWALIFFMMLATLGLDSSFGGSEAIITALSDEFPKIKRNRELFVAGLFSLYFVVGLASCTQGGFYFFHLLDRYAAGYSILVAVFFEAIAVSWIYGT--NRFSEDIRDMIGFPPGRYWQVCWRFVAPIFLLF---ITVYGLIGYEPLTYADYVYPSWANALGWCIAGSSVVMIPAVAI--------

General information: TITO was launched using: RESULT: Template: 4XP1.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 2626 -341403 -130.01 -724.85 target 2D structure prediction score : 0.59 Monomeric hydrophicity matching model chain A : 0.80 3D Compatibility (PKB) : -130.01 2D Compatibility (Sec. Struct. Predict.) : 0.59 1D Compatibility (Hydrophobicity) : 0.80 QMean score : 0.377

(partial model without unconserved sides chains): PDB file : Tito_4XP1.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4XP1-query.scw PDB file : Tito_Scwrl_4XP1.pdb: