Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MGITPMYVCLC------RGITDQDIKDAIENGAESYREIRDLLDLGT--CCGR-CAPEARAIISEELAEIAARISVAA 2GAG Chain:A ((475-538)) ----DAVNYKFHFVDLQRDQTVADVLRATGAGMKSVEHIKRYTSISTANDQGKTSGVAAIGVIAAVLG----------

General information: TITO was launched using: RESULT: Template: 2GAG.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 202 6533 32.34 118.78 target 2D structure prediction score : 0.55 Monomeric hydrophicity matching model chain A : 0.64 3D Compatibility (PKB) : 32.34 2D Compatibility (Sec. Struct. Predict.) : 0.55 1D Compatibility (Hydrophobicity) : 0.64 QMean score : 0.613

(partial model without unconserved sides chains): PDB file : Tito_2GAG.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2GAG-query.scw PDB file : Tito_Scwrl_2GAG.pdb: