Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence ---------------MTDIT--LKTPVEMRNVQLKVYDNTDNSILIDTQVASLTSYDIKLPSTKLNRLYRIEVTTQGSSQVFDPTKSEYQNISGVYHAFITPSSISNKTQLISPSSEAIYQRAVIRSGQLPNETIIPTRIEQLH---VDLA-SQDVYRSLLNAFKDTNIPSLSPASTLTILNLLKYSTTKPSTYVDSYLCFGYLQYWSSIHNTNSPYQDLTQSLATDLKDGYLDGKKLIGDKTSFTPIFTSAPDNIDPAKNTLLDIAANQKVTRDYFATNLRAATFKLADLQDQSHLDPIGYDL--LDKKQYSGIIPAGNTSEFFRIDGAGDYRRAVGFSGITATCNGSAYPCKQGLTSINISDPKLPATDYLVGHYEDSTTGCQLNFRANG-QIELIKGSQSYRSALSADSTDKLLQTDAASYSYLLNSSSSEPNNSTQQYNFIQLHIKANKIVSAQAGLDSRKAPDVLQTTQLEYSFS 4RAP Chain:A ((10-391)) ITPPDTPTQAGPENIFYDFNDGARVLLPEGKWHVRLLDADSENILFCCDVDK----GWVTSSKKYFVRFRIQVFRQGATPLLDET-----------------LKLKDRPVLISFPTG--------TLGDLLGWFPYAERFQSLHKCRLECTMSQDIIDLLAPQYPQ-----------------IQFSTPDKPRTVAPYATYRVGLYFGG--DTNNQPVDFRKVGFHR-SAGYILG---VDPREAPVRLDLSAPRVIAAP---YVCIATQSTCQAKYWNNGT-------GWSEVIAHLKSLGYRVMCIDRDAHYGQGFVWNHIPW----GAEDF---------------------TGKLPLQERVNLLRHASFFIG----LPSGLSWLAWATRIPVVLISG---FSLPNSEFYTPWRVFNSHGCYGCWDDTS---LNFDHHDFLWCPRHKNTDRQFECTRLITGAQVNGVINKLHRSLT--

General information: TITO was launched using: RESULT: Template: 4RAP.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1902 46235 24.31 129.51 target 2D structure prediction score : 0.43 Monomeric hydrophicity matching model chain A : 0.66 3D Compatibility (PKB) : 24.31 2D Compatibility (Sec. Struct. Predict.) : 0.43 1D Compatibility (Hydrophobicity) : 0.66 QMean score : 0.160

(partial model without unconserved sides chains): PDB file : Tito_4RAP.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4RAP-query.scw PDB file : Tito_Scwrl_4RAP.pdb: