Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNEKKQSRLRRAKSTRLHIRALGVTRLCVNRTPRHIYAQVISADGGKVLAQASTLDASL--RSGTTGNIEAATKVGALIAERAKAAGVTKVAFDRSGFKYHGRIKALADAAREGGLEF 5GAD Chain:P ((18-117)) ------------------LQELGATRLVVHRTPRHIYAQVIAPNGSEVLVAASTVEKAIAEQLKYTGNKDAAAAVGKAVAERALEKGIKDVSFDRSGFQYHGRVQALADAAREAGLQF

General information: TITO was launched using: RESULT: Template: 5GAD.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain P - contact count / total energy / energy per contact / energy per residue : 440 -13243 -30.10 -135.13 target 2D structure prediction score : 0.68 Monomeric hydrophicity matching model chain P : 0.87 3D Compatibility (PKB) : -30.10 2D Compatibility (Sec. Struct. Predict.) : 0.68 1D Compatibility (Hydrophobicity) : 0.87 QMean score : 0.774

(partial model without unconserved sides chains): PDB file : Tito_5GAD.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5GAD-query.scw PDB file : Tito_Scwrl_5GAD.pdb: