Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MLFYKNILTLSILAAISIPVFAAENENVEKLETIRIKAHPLEQTSKDFAVADTVVDQKHLTEGAAT-IGDALNSEVGIYANQFGAGS--SRPVIRGQDGPRVKVLQNSSENVDVSTLSPDHAVTVDPV-LAKQVEVIRGPSTLLFGAGTVGGLVNVIDNKIPTQIPENGYEGQVGLRYNTGSDEKLASAGVTVGLGSQVALRIEGLTRDANNYIAPNYIHEGEKERRVDNTFAQGDSVNVGLSWVYDRGYTGISYSNRRDQYGLPGHSHEYETCH---IHDLS-LHCGDHDHEGHSDEEAHDHEHEHGGPWID---LKSERYDFKTELNDPFAGFQKLRAQASYTDYQHDEIEEGTIATRFQNKGYDGRIELVHNPIADWEGVIGTQLGQQKLNLTGEEAFMAPTTTKKWS-VFALEHKQWKDVHFELSARADQQEIDVDDNSKQDFDGSAFSYAGAANWEFAPNYKLSFVASH--QERLPLAQELYANGGHFATNTYELGNDQLSKEKSNNVELGLHFDNDKLDYHLHVYHNWFDDYIYAQTLDRYKDFRLVQYTQDKARFYGAEGEIGYQITPMYKISAFGDYVRGKIDAEGNAPRIPAGRLGTKVDADFG--DGFSGSAEYYHVFNQDK-IAAYETET---EGYNMLNLGVAYSGQYGAKTDYRVYLKANNLLD---DTVYQHASFLSNIPQVGRNFTVGVDFSF 3RGM Chain:A ((7-594)) --------------------------------TLVVTANRFEQPRSTVLAPTTVVTRQDIDRWQSTSVNDVLRRLPGVDITQNGGSGQLSSIFIRGTNASHVLVLIDGVR-LNLAGVSGSADLSQFPIALVQRVEYIRGPRSAVYGSDAIGGVVN-----IITTRDEPGTE-------------------ISAGWGSN----------SYQNYDVSCQQQLGDKTR-----------VTLLGDYAHTHG------------------GFLSKTLYGALEHNFTDAWSGFVRGYGYDNRTN------------DTRKLYSQSWDAGLRYNGELIKSQLITSYSHSKDYNY--------ATLDEMKQY--TVQWANNVIVG-HGSIGAGVDWQKQTTTPGTGYVEDGYDQRNTGIYLTGLQQVGDFTFEGAARSD-------DNSQF---GRHGTWQTSAGWEFIEGYR--FIASYGTSYKAPNLGQLY---GFY-------GNPNLDPEKSKQWEGAFEGLTAGVNWRISGYRNDVSDLI------DYDDHTLKYYNEGKARIKGVEATANFDTGPLTHTVSY-DYVDAR-NAITDTPLLRRAKQQVKYQLDWQLYDFDWGITYQYLGTRYDKDYSSYPYQTVKMGGVSLWDLAVAYP----VTSHLTVRGKIANLFDKDYETVYGYQT-------AGREYTLSGSYTF

General information: TITO was launched using: RESULT: Template: 3RGM.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 3190 114216 35.80 217.55 target 2D structure prediction score : 0.44 Monomeric hydrophicity matching model chain A : 0.70 3D Compatibility (PKB) : 35.80 2D Compatibility (Sec. Struct. Predict.) : 0.44 1D Compatibility (Hydrophobicity) : 0.70 QMean score : 0.141

(partial model without unconserved sides chains): PDB file : Tito_3RGM.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3RGM-query.scw PDB file : Tito_Scwrl_3RGM.pdb: