Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSFVPQIKIPATYMRGGTSKGVFFKLDDLPEKAQVAGQARDQLLLRVIGSPDPYGKQIDGMGGATSSTSKTVILAKSTQPDHDVDYLFGQVSIDQAFVDWSGNCGNLTAAVGSFAISNGLVDADRIPENGLCTVRIWQKNIQKTIIAHVPITNGQVQETGDFELDGVTFPAAEVQIEFLDPADDGEEGGDMFPTGNVVDQLDVPEIGSFQATFINAGIPTIFLNAEDLGYEGTELQDHINGDAAALARFEKIRAYGAVQMGLIKDISEAAA-RQHTPKIAFVSKPKNYTASSGKNVS-ENDVDLLVRALSMGKLHHAMMGTAAVAIGTAAAIPGTLVNLAAGGG-EREAVRFGHPSGTLRVGAQAELTNGQWVVKKAIMSRSARVLMEGWVRIPGDSS 3G7K Chain:B ((2-378)) ---SDQMRIPCVIMRAGTSKGIFLKGNDLPAD----QELRDKVILRIFGSPDV--RQIDGLAGADPLTSKLAIIGPSTHPDADVDYTFAQVSITDAVVDYNGNCGNISAGVGPFAIDESFVKA----VEPMTRVCIHNTNTGKLLYAEVEVEDGKAKVSGDCKIDGVPGTNAPELMDFSDTAGA--ATGKVLPTGNVVDVLSTSK-GDIDVSIVDVANPCIFVHAKDVNMTGTETPDVINGNADLLAYLEEIRAKCCVKIGMAATEKEASEKSPAFPMIAFVTKPEDYVDFSTGNTISGDDVDLVSRLMFMQVLHKTYAGTATACTGSAARIPGTIVNQVLRDTGDEDTVRIGHPAGVIPVVSIVK----DGKVEKAALIRTARRIMEGYVYVEKAK-

General information: TITO was launched using: RESULT: Template: 3G7K.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 2203 -132722 -60.25 -354.87 target 2D structure prediction score : 0.59 Monomeric hydrophicity matching model chain B : 0.82 3D Compatibility (PKB) : -60.25 2D Compatibility (Sec. Struct. Predict.) : 0.59 1D Compatibility (Hydrophobicity) : 0.82 QMean score : 0.543

(partial model without unconserved sides chains): PDB file : Tito_3G7K.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3G7K-query.scw PDB file : Tito_Scwrl_3G7K.pdb: