Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MLLEFTKMHGLGNDFMVVDLISQRAYLDTATIQRLADRHFGVGFDQLL-IVEPPDVPEADFKYRIF-----NADGSEVEQCGNGVRCFARFVHERHLTNKTNITVQTKAGIVKPELGQ----NGWVRVNMGYPKFLPNEIPFVADEPEALYTLELANDQNISIDVVNMGNPHAVTIVPDVLTADVAGIGPQVESHKR--FPERINAGFMQVIDDKHVRLRVFERCVGETLACGTGAC-AAAVSGMRRGLLANSVEVELAGGKLQIEWQEGDVVWMTGPTTHVYDGRLDLRYFQG 5M47 Chain:A ((3-277)) LTIPFAKGHATENDFIIIPDEDARLDLTPEMVVTLCDRRAGIGADGILRVVKAADVEGSTVDPSLWFMDYRNADGSLAEMCGNGVRLFAHWLYSRGLVDNTSFDIGTRAGVRHVDILQADQHSAQVRVDMGIPDV------------TGLSTCDI-NGQVFAGLGVDMGNPHLACVVPGLSASALADMELRAPTFDQEFFPHGVNVEIVTELEDDAVSMRVWERGVGETRSCGTGTVAAACAALADAGLGEGTVKVCVPGGEVEVQIFD-DGSTLTGPSAIIALGEVQI-----

General information: TITO was launched using: RESULT: Template: 5M47.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1608 -46965 -29.21 -179.25 target 2D structure prediction score : 0.58 Monomeric hydrophicity matching model chain A : 0.78 3D Compatibility (PKB) : -29.21 2D Compatibility (Sec. Struct. Predict.) : 0.58 1D Compatibility (Hydrophobicity) : 0.78 QMean score : 0.426

(partial model without unconserved sides chains): PDB file : Tito_5M47.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5M47-query.scw PDB file : Tito_Scwrl_5M47.pdb: