Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MAALLGVNIDHVATLRQARGTAYPDPVEAALICEQAGAEGITLHLREDRRHIQDDDVRRMRPLLKTRMNLEMAVTDEMVEFAKEIQPQHVCFVPERRQEVTTEGGLDVVGNFEKVKAATQALAAIGCDVSLFIDADFAQIDAAIACGAPTIELHTGAYTDATTEQEQQEELARIVKGVEYAASKGLVVNAGHGLNLKNIAPIAAIPQIHALNIGHSIIAESVFVGLVQAVKDMKTAIQAAG 3F4N Chain:H ((8-244)) ---LLGVNIDHIATLRNARGTIYPDPVQAAFIAEQAGADGITVHLREDRRHITDRDVRILRQTIQTRMNLEMAVTDEMVDIACDIKPHFCCLVPEKRQEVTTEGGLDVAGQVDKMTLAVGRLADVGILVSLFIDADFRQIDAAVAAGAPYIEIHTGAYADASTVLERQAELMRIAKAATYAAGKGLKVNAGHGLTYHNVQPIAALPEMHELNIGHAIIGQAVMTGLAAAVTDMKVLMREA-

General information: TITO was launched using: RESULT: Template: 3F4N.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain H - contact count / total energy / energy per contact / energy per residue : 1357 -180872 -133.29 -763.17 target 2D structure prediction score : 0.74 Monomeric hydrophicity matching model chain H : 0.90 3D Compatibility (PKB) : -133.29 2D Compatibility (Sec. Struct. Predict.) : 0.74 1D Compatibility (Hydrophobicity) : 0.90 QMean score : 0.504

(partial model without unconserved sides chains): PDB file : Tito_3F4N.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3F4N-query.scw PDB file : Tito_Scwrl_3F4N.pdb: