Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MANQTPQAMRRIPVSDIEQRVKKAVAEQLGLKAEEIKNEASFMDDLGADSLDLVELVMSFENDFDITIPDEDSNEITTVQSAIDYVTKKLG 2FAC Chain:A ((1-72)) --------------STIEERVKKIIGEQLGVKQEEVTNNASFVEDLGADSLDTVELVMALEEEFDTEIPDEEAEKITTVQAAIDYI-----

General information: TITO was launched using: RESULT: Template: 2FAC.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 246 -32395 -131.69 -449.93 target 2D structure prediction score : 0.54 Monomeric hydrophicity matching model chain A : 0.87 3D Compatibility (PKB) : -131.69 2D Compatibility (Sec. Struct. Predict.) : 0.54 1D Compatibility (Hydrophobicity) : 0.87 QMean score : 0.616

(partial model without unconserved sides chains): PDB file : Tito_2FAC.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2FAC-query.scw PDB file : Tito_Scwrl_2FAC.pdb: