Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence -----------MKNKNIGCWLLLAGSSS--VCAMQPLDDQSLAAATGQNGLTLGIQADQVKFNQVAL-IDTNGIASTSYNSKAGLIIAGNSTNPVPGIEFIKAAVSTN--PSFNIAIDTDAGGGNPFLNLAVTMGSDVNGIR-LLPFSVYLAPSTSLPSPSDYALTSYAPKSIFSSGTTVNTGVKELIRSTGNLDINFVQTNKPRLNIQL--GHAAQSVMVKFGGAIQSICSAASGCPITLVSDNTGATFGFKFAGTNASTGFVLDGFYAGVDPTGLTIGNIGVSSKF-DASLNNVTLGNLGTQSTTTFNNLPNGSMGSFGVTGASVTDFKMKVSGF------- 3IGF Chain:A ((2-369)) ALILTFLGKSGVARTKIAIAAAKLLASQGKRVLLAGLAEPVLPLLLEQT---LTPDPQQIAPNLEVVQFQSSVLLERNWEEVKKLEAQYLRTPIIKEVYGQELVVLPGMDSALALNAIREYDASGKYDTIVYDGTGDAFTLRMLGLPESLSWYVRRFRQLF----TNQVNNFLDKGKEALADPKRVAAFLVTTADPLEVVSVRYLWGSAQQIGLTIGGVIQVSSQTEGDLSAEFTPLSVTVVPDVTKGDWQPLIDALPN----FVEQAEQAPKPITIDTHNRQVRLFLPGFDKKQVKLTQYGPEVTVEAGDQRRNIFLPPALSGRPITGAKFQNNYLIISFLEH

General information: TITO was launched using: RESULT: Template: 3IGF.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1491 5414 3.63 17.69 target 2D structure prediction score : 0.36 Monomeric hydrophicity matching model chain A : 0.63 3D Compatibility (PKB) : 3.63 2D Compatibility (Sec. Struct. Predict.) : 0.36 1D Compatibility (Hydrophobicity) : 0.63 QMean score : 0.112

(partial model without unconserved sides chains): PDB file : Tito_3IGF.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3IGF-query.scw PDB file : Tito_Scwrl_3IGF.pdb: