Modeling by threading (Tito software)
Unconserved sides chains calculation (Scwrl software)
Evaluation (QMean software)



Input alignment information:
Query sequence-----------MKNKNIGCWLLLAGSSS--VCAMQPLDDQSLAAATGQNGLTLGIQADQVKFNQVAL-IDTNGIASTSYNSKAGLIIAGNSTNPVPGIEFIKAAVSTN--PSFNIAIDTDAGGGNPFLNLAVTMGSDVNGIR-LLPFSVYLAPSTSLPSPSDYALTSYAPKSIFSSGTTVNTGVKELIRSTGNLDINFVQTNKPRLNIQL--GHAAQSVMVKFGGAIQSICSAASGCPITLVSDNTGATFGFKFAGTNASTGFVLDGFYAGVDPTGLTIGNIGVSSKF-DASLNNVTLGNLGTQSTTTFNNLPNGSMGSFGVTGASVTDFKMKVSGF-------
3IGF Chain:A ((2-369))ALILTFLGKSGVARTKIAIAAAKLLASQGKRVLLAGLAEPVLPLLLEQT---LTPDPQQIAPNLEVVQFQSSVLLERNWEEVKKLEAQYLRTPIIKEVYGQELVVLPGMDSALALNAIREYDASGKYDTIVYDGTGDAFTLRMLGLPESLSWYVRRFRQLF----TNQVNNFLDKGKEALADPKRVAAFLVTTADPLEVVSVRYLWGSAQQIGLTIGGVIQVSSQTEGDLSAEFTPLSVTVVPDVTKGDWQPLIDALPN----FVEQAEQAPKPITIDTHNRQVRLFLPGFDKKQVKLTQYGPEVTVEAGDQRRNIFLPPALSGRPITGAKFQNNYLIISFLEH


General information:
TITO was launched using:
RESULT:

Template: 3IGF.pdb
Alignment : align.pir
Tito was launched with SMD and SCWRL
Tito text output
Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1491 5414 3.63 17.69
target 2D structure prediction score : 0.36
Monomeric hydrophicity matching model chain A : 0.63

3D Compatibility (PKB) : 3.63
2D Compatibility (Sec. Struct. Predict.) : 0.36
1D Compatibility (Hydrophobicity) : 0.63
QMean score : 0.112

(partial model without unconserved sides chains):
PDB file : Tito_3IGF.pdb:





(Unconserved sides chains are recalculated) :
Sequence: align-3IGF-query.scw
PDB file : Tito_Scwrl_3IGF.pdb: