Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNRKDEHVSLAKAFHD----KQKNEFDFVRIIHNPLPQIAVSDVDLSTQAVGFTLSSPFYINAMTGGSEKTKKINQDLAIVAREADLMIATGSVSAALKVPSLADTYTIMRQEYPHGKIIANIG-----AGTSVERAKEAIRLFHADALQIHLNAPQELVMPEGDRDFTNWKV-LIQETQTAIDVPLIVKEVGFGMTRETLNDLAALGVHTVDISGRSGTSFTQIENARRSKR------ELSYLADWGQSTVSSLLEANEADTSMEILASGGIRNAYDIFKALCLGANAVGTSGTVLTHLMNHGVEETIILMKQWQEELRLLYTMVGATNTAALHQQSLIFSGPVKDWCEARGIDLVKYGRRTEKSVGQK 3B06 Chain:A ((5-361)) VNRKVEHVEIA-AFENVDGLSSSTFLNDVILVHQGFPGISFSEINTKTKFFRKEISVPVMVTGMTGGRNELGRINKIIAEVAEKFGIPMGVGSQRVAIEKAEARESFAIVRKVAPTIPIIANLGMPQLVKGYGLKEFQDAIQMIEADAIAVHLNPAQEVFQPEGEPEYQIYALEKLRDISKELSVPIIVKESGNGISMETAKLLYSYGIKNFDTSGQGGTNWIAIEMIRDIRRGNWKAESAKNFLDWGVPTAASIMEVRYSVPDSFLVGSGGIRSGLDAAKAIALGADIAGMALPVLKSAI-EGKESLEQFFRKIIFELKAAMMLTGSKDVDALKKTSIVILGKLKEWAEYRGINLSIY-----------

General information: TITO was launched using: RESULT: Template: 3B06.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 2127 57952 27.25 169.95 target 2D structure prediction score : 0.67 Monomeric hydrophicity matching model chain A : 0.78 3D Compatibility (PKB) : 27.25 2D Compatibility (Sec. Struct. Predict.) : 0.67 1D Compatibility (Hydrophobicity) : 0.78 QMean score : 0.517

(partial model without unconserved sides chains): PDB file : Tito_3B06.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3B06-query.scw PDB file : Tito_Scwrl_3B06.pdb: