Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNDLSEKVIVIMGASSGIGEATTKLLAEKGAKLVIAARREDRLKAIK----ESLPEAELYIQTADVRDFAQVQAVIDLAMEKFGRIDVLYNNAGI----MPTAPLVEGHRDEWQNMLDINIMGVLNGISAVLPIMEKQKSGHIISTDSVAGHVVYPDSAVYCGTKFAVRAIMEGLRQEQRQNNIKSTIISPGAVQTELYQTI-------SDKKAALELHEAQ--KEWGLTSEDIASAVAFAIETPDRMSVSNMIIRPTTQEV 1IY8 Chain:A ((11-258)) ---FTDRVVLITGGGSGLGRATAVRLAAEGAKLSLVDVSSEGLEASKAAVLETAPDAEVLTTVADVSDEAQVEAYVTATTERFGRIDGFFNNAGIEGKQNPTESFTAA---EFDKVVSINLRGVFLGLEKVLKIMREQGSGMVVNTASVGGIRGIGNQSGYAAAKHGVVGLTRNSAVEYGRYGIRINAIAPGAIWTPMVENSMKQLDPENPRKAAEEFIQVNPSKRYGEAPE-IAAVVAFLLS--DDASYVNATVVP-----

General information: TITO was launched using: RESULT: Template: 1IY8.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1262 -46173 -36.59 -199.88 target 2D structure prediction score : 0.67 Monomeric hydrophicity matching model chain A : 0.78 3D Compatibility (PKB) : -36.59 2D Compatibility (Sec. Struct. Predict.) : 0.67 1D Compatibility (Hydrophobicity) : 0.78 QMean score : 0.521

(partial model without unconserved sides chains): PDB file : Tito_1IY8.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1IY8-query.scw PDB file : Tito_Scwrl_1IY8.pdb: