Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MEKTVSAKIEDLKLDILKQAKVAMEHAVDKEDSAMVAAIAEILAHV- 2LVH Chain:A ((7-50)) MERVYQCLRCGLTF---RTKKQLIRHLVNTEKVNPLSIDYYYQSFSV

General information: TITO was launched using: RESULT: Template: 2LVH.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 141 -10696 -75.85 -248.73 target 2D structure prediction score : 0.37 Monomeric hydrophicity matching model chain A : 0.62 3D Compatibility (PKB) : -75.85 2D Compatibility (Sec. Struct. Predict.) : 0.37 1D Compatibility (Hydrophobicity) : 0.62 QMean score : 0.132

(partial model without unconserved sides chains): PDB file : Tito_2LVH.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2LVH-query.scw PDB file : Tito_Scwrl_2LVH.pdb: