Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MIIMSFCVACGHKTEQKIPLGDHKVRRVCTHCGNIHYENPKVICGALALWEDKVLLCRRAIEPRYGLWTLPAGYMELFETMEQGAARETREEAEAEIEIEQLYCMYNIPRI----GQIYVLFKAQLKDGLFGAGEESIECRLFEEHEIPWGELAFPSVEHTLRHYFEDRKQQVFPTHLETLGTRLDHTG 5ANT Chain:B ((21-159)) -------------------------------------GASRLYTLVLVLQPQRVLLGMKKRGFGAGRWNGFGGKVQEGETIEDGARRELQEESGLTVDALHKVGQIVFEFVGEPELMDVHVFCTDSIQGTPV-ESDEMRPCWFQLDQIPF-KDMWPDDSYWFPLLLQKKKFHGYFKFQ-----------

General information: TITO was launched using: RESULT: Template: 5ANT.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 611 -21235 -34.75 -157.30 target 2D structure prediction score : 0.47 Monomeric hydrophicity matching model chain B : 0.64 3D Compatibility (PKB) : -34.75 2D Compatibility (Sec. Struct. Predict.) : 0.47 1D Compatibility (Hydrophobicity) : 0.64 QMean score : 0.301

(partial model without unconserved sides chains): PDB file : Tito_5ANT.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5ANT-query.scw PDB file : Tito_Scwrl_5ANT.pdb: