Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence WFFKNLSRKDAERQLLAPGNTHGSFLIRESESTAGSFSLSVRDFDQNQGEVVKHYKIRNLDNGGFYISPRITFPGLHELVRHY 1O4F Chain:A ((7-89)) WYFGKITRRESERLLLNAENPRGTFLVRESETTKGAYCLSVSDFDNAKGLNVKHYKIRKLDSGGFYITSRTQFNSLQQLVAYY

General information: TITO was launched using: RESULT: Template: 1O4F.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 292 -38148 -130.64 -459.61 target 2D structure prediction score : 0.73 Monomeric hydrophicity matching model chain A : 0.89 3D Compatibility (PKB) : -130.64 2D Compatibility (Sec. Struct. Predict.) : 0.73 1D Compatibility (Hydrophobicity) : 0.89 QMean score : 0.712

(partial model without unconserved sides chains): PDB file : Tito_1O4F.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1O4F-query.scw PDB file : Tito_Scwrl_1O4F.pdb: