Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MWYNRAMKHFDTIVIGGGPAGMMATISSSFYGQKTLLIEKNRKLGKKLAGTGGGRCNVTNNGSLDNLLAGIPG-NGRFLYSVFSQFDNHDIINFFTENGVKLKVEDHGRVFPASDKSRTIIEALEKKITELGGQVATQIEIVSVKKV--DDQFV-LKSADQ-TFTCEKLIVTTGGKSYPSTGSTGFGHEIARHFKHTITDLEAAESPLLTDF---PHKALQGISLDDVTLSYGK-HVITHDLLFTHFGLSGPAALRMSSFVKGGEVLSLDVLPQLSE-KDLVTFLEENREKSLKNALKTLLPERLAEFFVQ-GY--PEKVKQLTEKEREQLVQSIKELKIPVTGKMSLAKSFVTKGGVSLKEINPKTLESKLVPGLHFAGEVMDINAHTGGFNITSALCTGWVAGSLHYD 2GQF Chain:A ((2-400)) ------SQYSENIIIGAGAAGLFCAAQLAKLGKSVTVFDNGKKIGRKILMSGGGFCNFTNLEV-TP--AHYLSQNPHFVKSALARYTNWDFISLVAEQGITYHEKELGQLFCDE-GAEQIVEMLKSECDKYGAKILLRSEVSQVERIQNDEKVRFVLQVNSTQWQCKNLIVATGGLSMPGLGATPFGYQIAEQFGIPVIPPRASLVPFTYRETDKFLTALSGISL-PVTITALCGKSFYNQLLFTHRGISGPAVLQISNYWQPTESVEIDLLPNHNVEEEINQAKQSSPKQMLKTILVRLLPKKLVELWIEQGIVQDEVIANISKVRVKNLVDFIHHWEFTPNGTEGYRTAEVTMGGVDTKVISSKTMESNQVSGLYFIGEVLDVTGWLGGYNFQWAWSSAYACALSISR

General information: TITO was launched using: RESULT: Template: 2GQF.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 2193 12966 5.91 33.59 target 2D structure prediction score : 0.57 Monomeric hydrophicity matching model chain A : 0.78 3D Compatibility (PKB) : 5.91 2D Compatibility (Sec. Struct. Predict.) : 0.57 1D Compatibility (Hydrophobicity) : 0.78 QMean score : 0.465

(partial model without unconserved sides chains): PDB file : Tito_2GQF.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2GQF-query.scw PDB file : Tito_Scwrl_2GQF.pdb: