Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MRIILLGPPGAGKGTQAQLICKRYNIPQISTGDMLRAAIREGTELGLKAKSVMESGGLVSDELIIGLVKERIAQPDCVNGCIFDGFPRTIPQAEALEKEG----ISIDHVIEIDVPDEEIVKRLSGRRQHPASGRVYHVVYNPPKV--EGKDDETGEDLVQRPDD-QEETIRKRLASYHTETEQL---VGFYQGRAASGENAPTYDKLDGLRTIEDVQKDLFNILDK 2XB4 Chain:A ((1-223)) MNILIFGPNGSGKGTQGNLVKDKYSLAHIESGGIFREHIGGGTELGKKAKEFIDRGDLVPDDITIPMVLETLES-KGKDGWLLDGFPRNTVQAQKLFEALQEKGMKINFVIEILLPREVAKNRIMGRRICKNNPNHPNNIFIDAIKPNGDVCRVCGGALSARADDQDEGAINKRHDIYYNTVDGTLAAAYYYKNMAA--KEGFVYIELDGEGSIDSIKDTLLAQLA-

General information: TITO was launched using: RESULT: Template: 2XB4.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 915 -26982 -29.49 -126.67 target 2D structure prediction score : 0.74 Monomeric hydrophicity matching model chain A : 0.76 3D Compatibility (PKB) : -29.49 2D Compatibility (Sec. Struct. Predict.) : 0.74 1D Compatibility (Hydrophobicity) : 0.76 QMean score : 0.546

(partial model without unconserved sides chains): PDB file : Tito_2XB4.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2XB4-query.scw PDB file : Tito_Scwrl_2XB4.pdb: