Modeling by threading (Tito software)
Unconserved sides chains calculation (Scwrl software)
Evaluation (QMean software)



Input alignment information:
Query sequenceMKVFDYEDIQLIPNKCIVNSRSECDTTVTLG-KHTFKMPVVPANMQTIIDETIAEFLAENGYFYIMHRFDE-AAR---IPFIKK---------MKKRGLITSISVGVKKEEYSFIEKLAEESLNPDYITIDIAHGHANSVIDMIQHIKKYLPETFVIAGNVGTPEAVRELENAGADATKVGIGPGKVCITKIKTGFGTGGWQLAALRWCAKA---ARKPIIADGGIRTHGDIAKSVRFGATMVMIGSLFAGHEESPGETKVENGIVYKEYFGSASEFQKGEKRNVEGKKIWIQHKGSLKDTLVEMQQDLQSSISYAGGRDLEAIRK-VDYVIVKNSIFNGDTI
4FEZ Chain:B ((12-366))-EALTFDDVLLVPAHSTV-LPNTADLRTRLTKNIALNIPMVSASMDTVTEARLAIALAQEGGIGFIHKNMSIEQQAAQVHQVKIFESKPNACKDEQGRLRVGAAVGAAPGNEERVKALVEAG--VDVLLIDSSHGHSEGVLQRIRETRAAYPHLEIIGGNVATAEGARALIEAGVSAVKVGIGPGSICTTRIVTGVGVP--QITAIADAAGVANEYGIPVIADGGIRFSGDISKAIAAGASCVMVGSMFAGTEEAPGEVILYQGRSYKAY-------------------GRIAYKGHLKEIIHQQMGGLRSCMGLTGSATVEDLRTKAQFVRISGA-------


General information:
TITO was launched using:
RESULT:

Template: 4FEZ.pdb
Alignment : align.pir
Tito was launched with SMD and SCWRL
Tito text output
Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 1745 -29116 -16.69 -99.37
target 2D structure prediction score : 0.65
Monomeric hydrophicity matching model chain B : 0.76

3D Compatibility (PKB) : -16.69
2D Compatibility (Sec. Struct. Predict.) : 0.65
1D Compatibility (Hydrophobicity) : 0.76
QMean score : 0.493

(partial model without unconserved sides chains):
PDB file : Tito_4FEZ.pdb:





(Unconserved sides chains are recalculated) :
Sequence: align-4FEZ-query.scw
PDB file : Tito_Scwrl_4FEZ.pdb: