Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSEVLDVVIVGGGLVGGLTALLLAQGGVQPTVLDAAPVLDVEKTLSVMNPRVLALSQATIHLLKTVNVWDDLARQMPYTGMQVWNLNGYGEINFGHESVQRPISEQALGSMVEPSVLNVAIQQKMLEQLTDYRTQVRVTCIEQ---GVGCWHIQLADG--TALKTKLLIGADGANSFVREQAFIDLDVLDYKQAAISCAIKTSKPHHYVARQ----IFLPTGPLAYLPMASLEESENGYWQSIVWTLPDDYADEYSALSDREFMQLL--TRESQHMLGEVLDVRSRAQFPLKAR-----AAKQYVKAGLALIGDAAHVIHPLAGQGVNIGCLDAAVLCDALLHDLGRGVWAHEQTLMRYEHRRKGQNDAMMHSMSAIGWLESSELFPLIWARNVGLKQVEQISFLKERFMQQANGLGALQNTQYSR--------------------------- 3E1T Chain:A ((5-503)) -PEVFDLIVIGGGPGGSTLASFVAMRGHRVLLLEREAFPRHQIGESL-------LPATVHGICAMLGLTDEMKRAGFPIKRGGTFRWGKEPEPWTFGFTRHPDDPYGFAYQVERARFDDMLLRNSERKGVDVRERHEVIDVLFEGERAVGVRYRNTEGVELMAHARFIVDASGNRTRVSQAVGERVYSRFFQNVALYGYFENGKRLPAPRQGNILSAAFQDGWFWYIPL-------SDTLTSVGAVVSREAAEAIKDGHEAALLRYIDRCPIIKEYLAPATRVTTGDYGEIRIRKDYSYCNTSFWKNGMALVGDAACFVDPVFSSGVHLATYSALLVARAINTCL-AGEMSEQRCFEEFERRYRREYGNFYQFLVAFYDMNQDTDSYFWSARKIINTEERANEAFVRLIAGRSNLDEPVFQSNFMQGFTREITELQHLAMFGLVPSRDGLAWA

General information: TITO was launched using: RESULT: Template: 3E1T.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 2260 -96211 -42.57 -244.19 target 2D structure prediction score : 0.55 Monomeric hydrophicity matching model chain A : 0.68 3D Compatibility (PKB) : -42.57 2D Compatibility (Sec. Struct. Predict.) : 0.55 1D Compatibility (Hydrophobicity) : 0.68 QMean score : 0.392

(partial model without unconserved sides chains): PDB file : Tito_3E1T.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3E1T-query.scw PDB file : Tito_Scwrl_3E1T.pdb: