Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MAYDDQNIFAKILRGELPAIKLYEDDQVLAFMDIMPQADGHALVIPKTPAVTLLDLPPEAAAYTIQIVQKIAKAMETALNLDGIVLMQLSGAAAGQTVPHVHFHLIPTNV-HQ-LG-KHAAQLGDQDKIKALAEKIKAAL 3KSV Chain:A ((9-146)) --MAANCIFCKIIKGDIPCAKVAETSKALAFMDINPLSRGHMLVIPKEHASCLHELGMEDAADVGVLLAKASRAVAGPDGSMQYNVLQNNGSLAHQEVPHVHFHIIPKTDEKTGLKIGWDTVKVASDELAEDAKRYSEAI

General information: TITO was launched using: RESULT: Template: 3KSV.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 491 -68543 -139.60 -507.73 target 2D structure prediction score : 0.65 Monomeric hydrophicity matching model chain A : 0.74 3D Compatibility (PKB) : -139.60 2D Compatibility (Sec. Struct. Predict.) : 0.65 1D Compatibility (Hydrophobicity) : 0.74 QMean score : 0.652

(partial model without unconserved sides chains): PDB file : Tito_3KSV.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3KSV-query.scw PDB file : Tito_Scwrl_3KSV.pdb: