Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MIISTITEAIGSTPLYRFDNQHYSMPKGSAIYAKLEYLNPGGSIKDRLGSYLIKEGFAQGKINEK-TTIIEPTAGNTGIGIALAALTYKLRTVFVVPEKFSLEKQQLMKALGAKIIHSPSEQGIIGAISKSKKLAEEISNSYLPLQFENKDNPAAYYHTLGPEIFHEIKENIHSFVAGIGSGGTFAGTSTFLKEKYPDIRIIGVEPEGS-VLNGGDPAPHEIEGIGVEFIPPFLSPLSINQIETISDVEGFNYTRQLAREQGLLVGSSSGAAFAAALREIRRL-PPGHRVVTIFPDAADRYLSKNIYL 2ISQ Chain:A ((3-307)) -IAKDVTELIGNTPLVYLNNVAEGC--VGRVAAKLEMMEPCSSVKDRIGFSMISDAEKKGLIKPGESVLIEPTSGNTGVGLAFTAAAKGYKLIITMPASMSTERRIILLAFGVELVLTDPAKGMKGAIAKAEEILAKTPNGYMLQQFENPANPKIHYETTGPEIWKGTGGKIDGFVSGIGTGGTITGAGKYLKEQNANVKLYGVEPVESAILSGGKPGPHKIQGIGAGFIPSVLNVDLIDEVVQVSSDESIDMARQLALKEGLLVGISSGAAAAAAIKLAQRPENAGKLFVAIFPSFGERYLSTVLFD

General information: TITO was launched using: RESULT: Template: 2ISQ.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1939 -5435 -2.80 -18.00 target 2D structure prediction score : 0.66 Monomeric hydrophicity matching model chain A : 0.83 3D Compatibility (PKB) : -2.80 2D Compatibility (Sec. Struct. Predict.) : 0.66 1D Compatibility (Hydrophobicity) : 0.83 QMean score : 0.494

(partial model without unconserved sides chains): PDB file : Tito_2ISQ.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2ISQ-query.scw PDB file : Tito_Scwrl_2ISQ.pdb: