Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNRFASKEEEKLYYEHKASEEEFLNWYQKQERPEYDKPSLTVDIVLMCYNKEEDQLKVLLIQRKGHPYRNSWALPGGFVQK--DESTGESVLRETKEETGVVISKENIEQLHTFSTPNRDPRGWVVTVSYLAFIGEEPL-IAGDDAKEVRWFTLERHSNKIHLSSGEVAISLDLVTGESSGKDTLAFDHSQIILKAFNRVVNKMEHEPQVLQVLGKDFTITEARKVFAKFLGIDYKTIDHSNFKKAML--QYFDEIGERPVGIGRPSKIYQLKPGHHE 3GZ5 Chain:A ((21-229)) -------------------------------------QLLTVDAVLFTYH--DQQLKVLLVQRSNHPFLGLWGLPGGFIDETCDESLEQTVLRKLAEKTAVVP--PYIEQLCTVGNNSRDARGWSVTVCYTALMSYQACQIQIASVSDVKWWPLADVL-----------------------QMPLAFDHLQLIEQARERLTQKALYSLVPGFALSEPFTLPELQHVHEVLLG---KPIQGKSFRRRVEQADLLIDTGLKRT----PANLYCLKPDT--

General information: TITO was launched using: RESULT: Template: 3GZ5.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 939 -26251 -27.96 -131.25 target 2D structure prediction score : 0.67 Monomeric hydrophicity matching model chain A : 0.74 3D Compatibility (PKB) : -27.96 2D Compatibility (Sec. Struct. Predict.) : 0.67 1D Compatibility (Hydrophobicity) : 0.74 QMean score : 0.528

(partial model without unconserved sides chains): PDB file : Tito_3GZ5.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3GZ5-query.scw PDB file : Tito_Scwrl_3GZ5.pdb: