Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MITKPDKNKTRQKRHRRVRNNISGTAERPRLNVFRSNKNIYAQIIDDVAGVTLASASTLDKEIS------GGTKTEAAQAVGKLVAERAAEKGIKVVVFDRGGYLYHGRVQALAEAARENGLEF 5MMI Chain:P ((46-166)) ---AHTRREDRTARHVRIRKKVEGTPERPRLCVFRSNKHLYVQVIDDTKMHTLAAASTMQKAISENIDYSAGPTVEVAQKIGEMIAKSCLEKGITKVAFDRGGYPYHGRVKALADAAREHGLVF

General information: TITO was launched using: RESULT: Template: 5MMI.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain P - contact count / total energy / energy per contact / energy per residue : 469 4380 9.34 38.09 target 2D structure prediction score : 0.69 Monomeric hydrophicity matching model chain P : 0.84 3D Compatibility (PKB) : 9.34 2D Compatibility (Sec. Struct. Predict.) : 0.69 1D Compatibility (Hydrophobicity) : 0.84 QMean score : 0.630

(partial model without unconserved sides chains): PDB file : Tito_5MMI.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5MMI-query.scw PDB file : Tito_Scwrl_5MMI.pdb: