Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSDWLDIAGKVVIVTGGSSGIGRSIVENLLKQNAQVANFDVTECR--I---------QHENLLSLKVDVSSKTDIEEGIYKVMKHFKTIDGLVNNAGINIPSLLIDRNHPKSKYELSEQVFDKMIAVNQKSVYLMSQAVGRILVQKGSGVIVNLSSESGLEGSEGQSCYAATKAAMNSFTRSWAKELGKRNVRVVGVAPGILEETGLRTQEYEEALSYTRGISVEQLRNGYSRTSTIPLGRSGKLQEVADLVCYYLSERSSYITGVTTNISGGKTRG 5U8P Chain:B ((61-309)) ----GRLVGRKTLVTGGDSGIGRAAAIAFAREGADVAIGYLPVEESDAREVVALIRAAGRQAVALPGDIRDETFCQRLVARAAEALGGLDILVNNAARQQALD--------SIGEMTTEHFDATVKTNLYGMFWITKAAIPHLPP--GASIINTTSVQAVRASANLLDYATTKAGIIAFTRSLAKQLGPRGIRVNAVAPGPYWTPLQ-----SSGGQP-----PETV-V--NYAAGSPYGRPGQPAEIAPLYVALAASETSYANGQVWGADGGLGI-

General information: TITO was launched using: RESULT: Template: 5U8P.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 1290 -11264 -8.73 -47.33 target 2D structure prediction score : 0.63 Monomeric hydrophicity matching model chain B : 0.71 3D Compatibility (PKB) : -8.73 2D Compatibility (Sec. Struct. Predict.) : 0.63 1D Compatibility (Hydrophobicity) : 0.71 QMean score : 0.491

(partial model without unconserved sides chains): PDB file : Tito_5U8P.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5U8P-query.scw PDB file : Tito_Scwrl_5U8P.pdb: