Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MAYDDQNIFAKILRGELPAIKLYEDDQVLAFMDIMPQADGHALVIPKTPAVTLLDLPPEAAAYTIQIVQKIAKAMETALNLDGIVLMQLSGAAAGQTVPHVHFHLIPTNV-HQLG-KHAAQLGDQDKIKALAEKIKAAL 3P0T Chain:A ((3-134)) --GSMASIFTKIINRELPGRFVYEDDDVVAFLTIEPMTQGHTLVVPREEIDNWQDVDSAAFNRVMGVSQLIGKAVCKAFRTERSGLI-----IAGLEVPHLHVHVFPTRSLSDFGFANVDRNPSPESLDEAQAKIKAAL

General information: TITO was launched using: RESULT: Template: 3P0T.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 577 -77180 -133.76 -593.69 target 2D structure prediction score : 0.69 Monomeric hydrophicity matching model chain A : 0.74 3D Compatibility (PKB) : -133.76 2D Compatibility (Sec. Struct. Predict.) : 0.69 1D Compatibility (Hydrophobicity) : 0.74 QMean score : 0.639

(partial model without unconserved sides chains): PDB file : Tito_3P0T.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3P0T-query.scw PDB file : Tito_Scwrl_3P0T.pdb: