Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTSLIEKQIVNKEIRVTDPLFEVIHQIQAENEEPLAALNTGYHEPADIRKRLENIISDKVDDTVTVLLPFYTDFGKHISIGKNVFINRQVMFVDLGGICLEDSVLIGPRVNLITVNHLTDPKER--RGLSVKPIHIKKNAWIGAGATILPGVTIGENAIVAANATVTKDVPDNTIVAGIPAKIVKPVERII 3NZ2 Chain:J ((7-187)) --SELEKMLKGEHFDGASAE---IEALRSQAGRLKLEINQSL-DEAERYALQRELFG-HLGHKSCVQPPFHCEFGKTIRIGDHTFINMNVVMLDGAPITIGDHVLIGPSTQFYTASHSLDYRRRQAWETICKPIVIEDDVWIGGNVVINQGVTIGARSVVAANSVVNQDVPPDTLVGGTPARILRSLK---

General information: TITO was launched using: RESULT: Template: 3NZ2.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain J - contact count / total energy / energy per contact / energy per residue : 930 -84849 -91.24 -474.02 target 2D structure prediction score : 0.56 Monomeric hydrophicity matching model chain J : 0.78 3D Compatibility (PKB) : -91.24 2D Compatibility (Sec. Struct. Predict.) : 0.56 1D Compatibility (Hydrophobicity) : 0.78 QMean score : 0.633

(partial model without unconserved sides chains): PDB file : Tito_3NZ2.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3NZ2-query.scw PDB file : Tito_Scwrl_3NZ2.pdb: