Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MIRTMQASDIDSVAQIEKLVQTHPWSRQQFVESLN-SYQCTVIELNNKVVGFCILQPVLDEANLLLMAIDPQMQGKGLGYQLLDASIERLEN-HPVQIFLEVRESNKAAIGLYEKAGFHQIDVRRNYYPTQEGGHENAIIMVKSCTDDFASLF 5ISV Chain:B ((3-150)) TISSLETTDLPAAYHIEQRAHAFPWSEKTFASNQGERYLNFQLTQNGKMAAFAITQVVLDEATLFNIAVDPDYQRQGLGRALLEHLIDELEKRGVATLWLEVRASNAAAIALYESLGFNEATIRRNYYPT-TDGREDAIIMALPISMAG----

General information: TITO was launched using: RESULT: Template: 5ISV.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 635 -36819 -57.98 -252.18 target 2D structure prediction score : 0.66 Monomeric hydrophicity matching model chain B : 0.78 3D Compatibility (PKB) : -57.98 2D Compatibility (Sec. Struct. Predict.) : 0.66 1D Compatibility (Hydrophobicity) : 0.78 QMean score : 0.633

(partial model without unconserved sides chains): PDB file : Tito_5ISV.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5ISV-query.scw PDB file : Tito_Scwrl_5ISV.pdb: