Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSIYNNITELIGQTPIVKLNNIVPEGAADVYIKLEAFNPGSSVKDRIALSMIEKAEQDGILKLGSTIVEATSGNTGIGLSWVGAAKGYKVVIVMPETMSVERRKIIQAYGAELVLTPGSEGMKGAIAKAQEIAAERDGFLPL-QFDNPANPEVHERTTGAEILAAFGKD-GLDAFVAGVGTGGTISGVSHALKSENSNIQVFAVEADESA-ILSGEKPGPHKIQGISAGFIPDTLDTKAYDGIVRVTSDDALALGREIGGKEGFLVGISSAAAIYGAIEVAKKL-GTGKKVLALAPDNGERYLSTALYEL 2Q3D Chain:A ((4-309)) MSIAEDITQLIGRTPLVRLRRVTDGAVADIVAKLEFFNPANSVKDRIGVAMLQAAEQAGLIKPDTIILEPTSGNTGIALAMVCAARGYRCVLTMPETMSLERRMLLRAYGAELILTPGADGMSGAIAKAEELAKTDQRYFVPQQFENPANPAIHRVTTAEEVWRDT--DGKVDIVVAGVGTGGTITGVAQVIKERKPSARFVAVEPAASPVLSGGQ-KGPHPIQGIGAGFVPPVLDQDLVDEIITVGNEDALNVARRLAREEGLLVGISSGAATVAALQVARRPENAGKLIVVVLPDFGERYLSTPLFA-

General information: TITO was launched using: RESULT: Template: 2Q3D.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1932 -36837 -19.07 -121.98 target 2D structure prediction score : 0.65 Monomeric hydrophicity matching model chain A : 0.89 3D Compatibility (PKB) : -19.07 2D Compatibility (Sec. Struct. Predict.) : 0.65 1D Compatibility (Hydrophobicity) : 0.89 QMean score : 0.581

(partial model without unconserved sides chains): PDB file : Tito_2Q3D.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2Q3D-query.scw PDB file : Tito_Scwrl_2Q3D.pdb: