Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSENQQALNHVVSMEDLTVDQVMKLIKRGIEFKNGAQIPYEDHPIVSNLFFEDSTRTHKSFEVAEIKLGLERLDFDVKTSSVNKGETLYDTILTLSALGVDVCVIRHPEV-DYYRELIASPTITTSIINGGDGSGQHPSQSLLDLMTIYEEFGHFEGLKVAIAGDLDHSRVAKSNMQILKRLGSELFFAGPEEWRSQEFA--DYGKFVTIDEIIDQVDVMMFLRVQHERHDSGAVFSKEDYHAQHGLTQERYDRLKETAILMHPAPINRDVEIADHLVEAPKSRIVQQMTNGVFVRMAILESVLASRNAN 4BJH Chain:B ((32-321)) -------MRSLISSLDLTREEVEEILKYAKEFKEGK-EETI-KASAVLFFSEPSTRTRLSFEKAARELGIETYLVSGSESSTVKGESFFDTLKTFEGLGFDYVVFRVPFVFF--PYKEIVKSLNLRLVNAGDGTHQHPSQGLIDFFTIKEHFGEVKDLRVLYVGDIKHSRVFRSGAPLLNMFGAKIGVCGPKTLIPRDVEVFKVDVFDDVDKGIDWADVVIWLRLQKERQKENYIPSESSYFKQFGLTKERFEK---VKLYMHPGPVNRNVDIDHELVYTEKSLIQEQVKNGIPVRKAIYKFLW------

General information: TITO was launched using: RESULT: Template: 4BJH.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 1607 -32059 -19.95 -111.70 target 2D structure prediction score : 0.55 Monomeric hydrophicity matching model chain B : 0.78 3D Compatibility (PKB) : -19.95 2D Compatibility (Sec. Struct. Predict.) : 0.55 1D Compatibility (Hydrophobicity) : 0.78 QMean score : 0.468

(partial model without unconserved sides chains): PDB file : Tito_4BJH.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4BJH-query.scw PDB file : Tito_Scwrl_4BJH.pdb: