Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MQRNHICNIGGKNVGKPIISFDQVVKRYDD-ETVLKKVSFEIEQGKFYTLLGPSGCGKTTILRIIAGFTEATEGDIYFEGKRLNDLPAN--KRQVNTVFQDYALFPHMNVFDNVAFGLKIKKMSKSDIEKKVKDALR---MVQLPGYEKREISE----MSGGQRQRVAIARAIVNEPKVLLLDEPLSALDLKLRTDMQYELRDLQQRLGITFIFVTHDQEEALAMSDEIFVMNKGKIVQSGTPVDIYDEPINHFVADFVGESNIVDGVMIEDNLVEFVGKQFECVDGGMRLNEPVEVVLRPEDLTITTPDKGKLVVTVDTQLFRGVHYEIICYDEQQNEWMVHSTKKAKEGSKVGLAFEPEDIHVMRFNESEEEFDARLDSYEE 1MV5 Chain:D ((11-219)) ---------------------------YDDSEQILRDISFEAQPNSIIAFAGPSGGGKSTIFSLLERFYQPTAGEITIDGQPIDNISLENWRSQIGFVSQDSAIMAG-TIRENLTYGLE-GDYTDEDLWQVLDLAFARSFVENMPDQLNTEVGERGVKISGGQRQRLAIARAFLRNPKILMLDEATASLDSESESMVQKALDSLMK--GRTTLVIAH-RLSTIVDADKIYFIEKGQITGSG-----------------------------------------------------------------------------------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 1MV5.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain D - contact count / total energy / energy per contact / energy per residue : 810 20884 25.78 104.94 target 2D structure prediction score : 0.62 Monomeric hydrophicity matching model chain D : 0.69 3D Compatibility (PKB) : 25.78 2D Compatibility (Sec. Struct. Predict.) : 0.62 1D Compatibility (Hydrophobicity) : 0.69 QMean score : 0.487

(partial model without unconserved sides chains): PDB file : Tito_1MV5.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1MV5-query.scw PDB file : Tito_Scwrl_1MV5.pdb: