Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKVFDYEDIQLIPNKCIVNSRSECDTTVTLG-KHTFKMPVVPANMQTIIDETIAEFLAENGYFYIMHRFDE-AAR---IPFIKK--------------MKKRGLITSISVGVKKEEYSFIEKLAEESLNPDYITIDIAHGHANSVIDMIQHIKKYLPETFVIAGNVGTPEAVRELENAGADATKVGIGPGKVCITKIKTGFGTGGWQLAALRWCAKAA---RKPIIADGGIRTHGDIAKSVRFGATMVMIGSLFAGHEESPGETKVENGIVYKEYFGSAS-EFQKGEKRNVEGKKIWIQHKGSLKDTLVEMQQDLQSSISYAGGRDLEAIRK-VDYVIVKNSIFNGDTI 5AHM Chain:A ((28-385)) --ALTFDDVLLIPG-YSEVLPKDVSLKTRLTRGIELNIPLVSAAMDTVTEARLAIAMAQEGGIGIIHKNMGIEQQAAEVRKVKKHETAKAKTYPLASKDEQGRLRVGAAVGTGADTGERVAALVAAG--VDVVVVDTAHGHSKGVIERVRWVKQTFPDVQVIGGNIATAEAAKALAEAGADAVKVGIGPGSICTTRIVAGVGVP--QISAIANVAAALEGTGVPLIADGGIRFSGDLAKAMVAGAYCVMMGSMFAGTEEAPGEIELFQGRSYKSYRGMGSL--------VPEGIEGRVPYKGALSAIVHQLMGGLRAAMGYTGSADIQQMRTQPQFVRITGAGMAESHV

General information: TITO was launched using: RESULT: Template: 5AHM.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1915 6288 3.28 20.28 target 2D structure prediction score : 0.67 Monomeric hydrophicity matching model chain A : 0.75 3D Compatibility (PKB) : 3.28 2D Compatibility (Sec. Struct. Predict.) : 0.67 1D Compatibility (Hydrophobicity) : 0.75 QMean score : 0.489

(partial model without unconserved sides chains): PDB file : Tito_5AHM.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5AHM-query.scw PDB file : Tito_Scwrl_5AHM.pdb: