Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence PEISREQAIALLKDQEPGAFIIRDSHSFRGAYGLAMKVSSPPPTIMQQNKKGDMTHELVRHFLIETGPR------------------GVKLKGCPNEPNFGSLSALVYQH 2EYZ Chain:A ((16-104)) GRLSRQEAVALLQGQRHGVFLVRDSSTSPGDYVLSVSEN-----------------SRVSHYIINSSGPRPPVPPSPAQPPPGVSPSRLRI-G---DQEFDSLPALLEFY

General information: TITO was launched using: RESULT: Template: 2EYZ.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 254 -10373 -40.84 -146.10 target 2D structure prediction score : 0.65 Monomeric hydrophicity matching model chain A : 0.73 3D Compatibility (PKB) : -40.84 2D Compatibility (Sec. Struct. Predict.) : 0.65 1D Compatibility (Hydrophobicity) : 0.73 QMean score : 0.335

(partial model without unconserved sides chains): PDB file : Tito_2EYZ.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2EYZ-query.scw PDB file : Tito_Scwrl_2EYZ.pdb: