Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSENQQALNHVVSMEDLTVDQVMKLIKRGIEFKNGAQIPYEDHPIVSNLFFEDSTRTHKSFEVAEIKLGLERLDFDVKT--SSVNKGETLYDTILTLSALGVDVCVIRHPEVDYYRELIASPT-ITTSIINGGDGSGQHPSQSLLDLMTIYEEFGHFEGLKVAIAGDLDHSRVAKSNMQILKRL-GSELFFAGPEEW-RSQEFAD--------YGKFVTIDEIIDQVDVMMFLRVQHERHDSGAVFSKEDYHAQHGLTQERYDRLKETAILMHPAPINRDVEIADHLVEAPKSRIVQQMTNGVFVRMAILESVLASRNAN 4E2F Chain:A ((2-307)) ---NPLYQKHIISINDLSRDDLNLVLATAAKLKANPQPELLKHKVIASCFFEASTRTRLSFETSMHRLGASVVGFSDSANTSLGKKGETLADTISVISTY-VDAIVMRHPQEG--AARLATEFSGNVPVLNAGDGSNQHPTQTLLDLFTIQETQGRLDNLHVAMVGDLEYGRTVHSLTQALAKFDGNRFYFIAPDALAMPQYILDMLDEKGIAWSLHSSIEEVMAEVDILYMTRVQKERLDP---SKYANVKAQFVLRASDLHNAKANMKVLHPLPRVD--EIATDVDKTPHAWYFQQAGNGIFARQALLALVLNRD---

General information: TITO was launched using: RESULT: Template: 4E2F.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1457 -7997 -5.49 -27.29 target 2D structure prediction score : 0.56 Monomeric hydrophicity matching model chain A : 0.76 3D Compatibility (PKB) : -5.49 2D Compatibility (Sec. Struct. Predict.) : 0.56 1D Compatibility (Hydrophobicity) : 0.76 QMean score : 0.429

(partial model without unconserved sides chains): PDB file : Tito_4E2F.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4E2F-query.scw PDB file : Tito_Scwrl_4E2F.pdb: