Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MIISTITEAIGSTPLYRFDNQHYSMPKGSAIYAKLEYLNPGGSIKDRLGSYLIKEGFAQGKINEK-TTIIEPTAGNTGIGIALAALTYKLRTVFVVPEKFSLEKQQLMKALGAKIIHSPSEQGIIGAISKSKKLAEEISNSYLPLQFENKDNPAAYYHTLGPEIFHEIKENIHSFVAGIGSGGTFAGTSTFLKEKYPDIRIIGVEPEGS-VLNGGDPAPHEIEGIGVEFIPPFLSPLSINQIETISDVEGFNYTRQLAREQGLLVGSSSGAAFAAALREIRRL-PPGHRVVTIFPDAADRYLSKNIYL 1Z7W Chain:A ((5-309)) -IAKDVTELIGNTPLVYLNNVAEGC--VGRVAAKLEMMEPCSSVKDRIGFSMISDAEKKGLIKPGESVLIEPTSGNTGVGLAFTAAAKGYKLIITMPASMSTERRIILLAFGVELVLTDPAKGMKGAIAKAEEILAKTPNGYMLQQFENPANPKIHYETTGPEIWKGTGGKIDGFVSGIGTGGTITGAGKYLKEQNANVKLYGVEPVESAILSGGKPGPHKIQGIGAGFIPSVLNVDLIDEVVQVSSDESIDMARQLALKEGLLVGISSGAAAAAAIKLAQRPENAGKLFVAIFPSFGERYLSTVLFD

General information: TITO was launched using: RESULT: Template: 1Z7W.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1909 -4908 -2.57 -16.25 target 2D structure prediction score : 0.66 Monomeric hydrophicity matching model chain A : 0.83 3D Compatibility (PKB) : -2.57 2D Compatibility (Sec. Struct. Predict.) : 0.66 1D Compatibility (Hydrophobicity) : 0.83 QMean score : 0.486

(partial model without unconserved sides chains): PDB file : Tito_1Z7W.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1Z7W-query.scw PDB file : Tito_Scwrl_1Z7W.pdb: