Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MIRTMQASDIDSVAQIEKLVQTHPWSRQQFVESLN--SYQCTVIELNNKVVGFCILQPVL------------DEANLLLMAIDPQMQGKGLGYQLLDASIERLEN--HPVQIFLEVRESNKAAIGLYEKAGFHQIDVRRNYYPTQEGGHENAIIMVKSCTDDFASLF 4LX9 Chain:A ((13-166)) TLRNARMDDIDQIIKINRLTLPENYPYYFFVEHLKEYGLAFFVAIVDNSVVGYIMPRIEWGFSNIKQLPSLVRKGHVVSIAVLEEYRRKGIATTLLEASMKSMKNDYNAEEIYLEVRVSNYPAIALYEKLNFKKVKVLKGYYADG----EDAYLMARP---------

General information: TITO was launched using: RESULT: Template: 4LX9.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 584 -35912 -61.49 -260.23 target 2D structure prediction score : 0.71 Monomeric hydrophicity matching model chain A : 0.75 3D Compatibility (PKB) : -61.49 2D Compatibility (Sec. Struct. Predict.) : 0.71 1D Compatibility (Hydrophobicity) : 0.75 QMean score : 0.670

(partial model without unconserved sides chains): PDB file : Tito_4LX9.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4LX9-query.scw PDB file : Tito_Scwrl_4LX9.pdb: