Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence WNHGNITRSKAEELLSRTGKDGSFLVRASESISRAYALCVLYRNCVYTYRILPNEDDKFTVQASEGVSMRFFTKLDQLIEFY 5I6V Chain:B ((6-109)) WFHPNITGVEAENLLLTRGVDGSFLARPSKSNPGDFTLSV-RRNGAVTHIKIQNTGDYYDLYGGEK-----FATLAELVQYY

General information: TITO was launched using: RESULT: Template: 5I6V.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 289 -42774 -148.01 -562.81 target 2D structure prediction score : 0.62 Monomeric hydrophicity matching model chain B : 0.74 3D Compatibility (PKB) : -148.01 2D Compatibility (Sec. Struct. Predict.) : 0.62 1D Compatibility (Hydrophobicity) : 0.74 QMean score : 0.565

(partial model without unconserved sides chains): PDB file : Tito_5I6V.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5I6V-query.scw PDB file : Tito_Scwrl_5I6V.pdb: