Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MITKPDKNKTRQKRHRRVRNNISGTAERPRLNVFRSNKNIYAQIIDDVAGVTLASASTLDKEIS--GGTKTEAAQAVGKLVAERAAEKGIKVVVFDRGGYLYHGRVQALAEAARENGLEF 5O60 Chain:P ((11-127)) ---SEVRRNARLRRHARLRKKVAGTAEVPRLVVNRSARHIHVQLVNDLNGTTLAAASSIEADVRAIDGDKKAHSVRVGQLIAERAKAAGVETVVFDRGGYTYGGRIAALADAAREAGLKF

General information: TITO was launched using: RESULT: Template: 5O60.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain P - contact count / total energy / energy per contact / energy per residue : 476 -3401 -7.14 -29.57 target 2D structure prediction score : 0.68 Monomeric hydrophicity matching model chain P : 0.81 3D Compatibility (PKB) : -7.14 2D Compatibility (Sec. Struct. Predict.) : 0.68 1D Compatibility (Hydrophobicity) : 0.81 QMean score : 0.703

(partial model without unconserved sides chains): PDB file : Tito_5O60.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5O60-query.scw PDB file : Tito_Scwrl_5O60.pdb: