Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MRYPILIHPNDKLKRTAQPIDVITDETIALLDNLYETMIANDGIGIAAPQVGQNKRIAVIEVDEGEKFELINPEIIEAKGES-LDVEGCLSIPHVYGTVKRADEVTVRYYDRDGEEIEVTAFGYLARAFQHEIDHLDGILFIEKMIQQIPEEELEEYMEEHLDD 1RL4 Chain:B ((10-172)) ---KIVKYPDPILRRRSEEVTNFDDNLKRVVRKMFDIMYESKGIGLSAPQVNISKRIIVWN------RIFINPSIVEQSLVKLKLIEGCLSFPGIEGKVERPSIVSISYYDINGYKHLKILKGIHSRIFQHEFDHLNGTLFIDKMTQVDKKKVRPKLNELI---

General information: TITO was launched using: RESULT: Template: 1RL4.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 728 -30454 -41.83 -201.68 target 2D structure prediction score : 0.61 Monomeric hydrophicity matching model chain B : 0.71 3D Compatibility (PKB) : -41.83 2D Compatibility (Sec. Struct. Predict.) : 0.61 1D Compatibility (Hydrophobicity) : 0.71 QMean score : 0.455

(partial model without unconserved sides chains): PDB file : Tito_1RL4.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1RL4-query.scw PDB file : Tito_Scwrl_1RL4.pdb: