Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MEAQEIIRYIQTASKKTPVKVYLNVTEPIEFKESKVFGEGSSFVVFGDYETIAPVLEAESEKIIEIEIETAARYSAVPLLDIKKINARIEPGAIIRDQVSIGNNAVIMMGAIINIGAVIGENTMIDMGAVLGGRATVGKNCHIGAGAVLAGVIEPASAKPVIVEDGVLVGANAVIVEGVHIGKDAVVAAGAVVLE---------------DVAAETVVGG--IPARVL---------------KISDDQTKENTALIAALREL 3EG4 Chain:A ((89-284)) --------------------------------------------WWDKVPSKFDGWTANEFEKAGF---------------------RAVPNCIVRHSAYIAPNAILMPS-FVNLGAYVDKGAMIDTWATVGSCAQIGKNVHLSGGVGIGGVLEPMQAGPTIIEDNCFIGARSEVVEGCIVREGSVLGMGVFIGKSTKIVDRATGEVFYGEVPPYSVVVAGTMPGKNVPGENWGPSLYCAVIVKRADEKTRSKTSINELLRD-

General information: TITO was launched using: RESULT: Template: 3EG4.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 874 -135550 -155.09 -826.52 target 2D structure prediction score : 0.31 Monomeric hydrophicity matching model chain A : 0.68 3D Compatibility (PKB) : -155.09 2D Compatibility (Sec. Struct. Predict.) : 0.31 1D Compatibility (Hydrophobicity) : 0.68 QMean score : 0.485

(partial model without unconserved sides chains): PDB file : Tito_3EG4.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3EG4-query.scw PDB file : Tito_Scwrl_3EG4.pdb: