Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MANRGNVHQLLKSKEEIEYLVTLHTHRYANMPLPKQLEKKFWCLNLDRTRQNISKFLASGVLTYLIFILLALPTDYLVIGVPYITLDFIHCLLSAMNIALALLLFWVFAKFTKLSEHFYLAACGIVFLTIIINAMLLLSVGNMALKNQSMLLLSFLYMLGFILSGIKPLHMLCVGLLAAFLVFAFLILLDVNCDYIALGRALFGSCILGFSISSMLISRERSLFLNNQLAEINEQILRIEASELLHLSQQDALTQISNRRTFDEMFDFFYYRANQEKRPLAVLFIDIDFFKNYSDFTGTRWEIKSSLALPQQLKTQFVMSTLLRVMGARSLLYYYLKHLHRVLIQWLQISIKPLSGKRFLMQLL 4ZVH Chain:A ((2-49)) ----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------DVLTKLLNRRFLPTIFKREIAHANRTGTPLSVLIIDVDKFKEINDTWG------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 4ZVH.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 20 -3912 -195.58 -81.49 target 2D structure prediction score : 0.58 Monomeric hydrophicity matching model chain A : 0.44 3D Compatibility (PKB) : -195.58 2D Compatibility (Sec. Struct. Predict.) : 0.58 1D Compatibility (Hydrophobicity) : 0.44 QMean score : 0.146

(partial model without unconserved sides chains): PDB file : Tito_4ZVH.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4ZVH-query.scw PDB file : Tito_Scwrl_4ZVH.pdb: