Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTAQMKTNASAKKAVNSPNHIYDTFIVGAGISGIAAAIRLDQVGYTNYKIIEKAGRVGGTWRENTYPGCGCDVPSALYSYSFAPSA----KWGHLFARQPEILSYLEDVSNEFNITSKIEFYNELLNAAWDESRHLWVLDTTTGQYL-SRTVIFATGPITEAQIPRLEGLDTFTGEMFHSAKWNHD-YDLTGKRIAVIGTGASAIQFVPQIQPKAKELFVFQRTAPWVLPKPDTDLG-ELSKSIIAKY---------------------PAIQAS-----------WRKSVALTLNAINFGLRNPLALKPVNVLGKQLLKLQIADPELRKNVTP-NFDIGCKRILFANNYYP-----------ALQAPNTTLIPHGLVKVEGNTVIAANGERHEVDVIIWGTGFEVSHPPIGKRVFNEKGQ-RLNDLWKNSSPEAYLGTNIENVPNAFLVLGP-NVLVYDSFIGLAEAQLDYIVDGLLKIKNKGISKLNVKADVIKKHNDLVQKHLQTTVFNS--GGCKSYYLDAN----GRNFAAWPWSLKKLKQRLKKLDLKDYEVTYQMSKTH 4D03 Chain:A ((13-537)) -----------------PPEEVDVLVVGAGFSGLYALYRLRELGRSVH-VIETAGDVGGVWYWNRYPGARDDIESIEYCYSFSEEVLQEWNWTERYASQPEILRYINFVADKFDLRSGITFHTTVTAAAFDEATNTWTVDTNHGDRIRARYLIMASGQLSVPQLPNFPGLKDFAGNLYHTGNWPHEPVDFSGQRVGVIGTGSSGIQVSPQIAKQAAELFVFQRTPHFAVPARNAPLDPEFLADLKKRYAEFREESRNTPGGTHRYQGPKSALEVSDEELVETLERYWQEGGPDILAAYRDILRDRDANERVAEFIRNKIRNTVRDPEVAERLVPKGYPFGTKRLILEIDYYEMFNRDNVHLVDTLSAPIETITPRGVRTSE---------REYELDSLVLATGFDALTGALFKIDIRGVGNVALKEKWA-AGPRTYLGLSTAGFPNLFFIAGPGSPSALSNMLVSIEQHVEWVTDHIAYMFKNGLTRSEA---VLEKEDEWVE-HVNEIADETLYPMTASWYTGANVPGKPRVFMLYVGGFHRYRQICDEVAAKGYE---------

General information: TITO was launched using: RESULT: Template: 4D03.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 2623 12719 4.85 27.29 target 2D structure prediction score : 0.64 Monomeric hydrophicity matching model chain A : 0.77 3D Compatibility (PKB) : 4.85 2D Compatibility (Sec. Struct. Predict.) : 0.64 1D Compatibility (Hydrophobicity) : 0.77 QMean score : 0.460

(partial model without unconserved sides chains): PDB file : Tito_4D03.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4D03-query.scw PDB file : Tito_Scwrl_4D03.pdb: