Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MVAQAIRNIISPKSVIAQYQKIDFSNKGTIPIRHLNIGFDGKEIDIEFYVNNQFATLFFATLSVFLTYGEDLVIETARHHREFIQDPILKQRVTSLIGQEAIHSKLHNEFNDAIKELEYPVDLYRFLGENFFKYVFLKFPQPLKLSLMAGIEHFTAVLAEYMMKHEKNFYYTDDAKSRALWMWHMLEESEHKDIAYDVYQILNGNYPLRATGFLMAFITILGLIPTATLLVPVLRKPKELLTRKFWKDARRGVNLIFSPKDGVFGSTIGQIFDYLRPNFHPNDHDTTQYLEYYKNKLLNEGGAIAPYFVKEFTPPVRAVS 4ETR Chain:A ((19-147)) --------------------------------------------------------------------TSKDGEAGFHACAEDLRDPQLKAAMLEQSRDCAAAADE---LERIVLELGGKP-------------------EEAVLNECERGEDVAKHRYQAALE-----K-SLPAEIHQVIERQYQGVLRHHDRVRALRD------------------------------------------------------------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 4ETR.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 336 -18308 -54.49 -176.04 target 2D structure prediction score : 0.69 Monomeric hydrophicity matching model chain A : 0.51 3D Compatibility (PKB) : -54.49 2D Compatibility (Sec. Struct. Predict.) : 0.69 1D Compatibility (Hydrophobicity) : 0.51 QMean score : 0.310

(partial model without unconserved sides chains): PDB file : Tito_4ETR.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4ETR-query.scw PDB file : Tito_Scwrl_4ETR.pdb: