Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MHSFVLVVHIILAVLMIALILVQHGKGADAGASFGGGGAATVFGASGSGNF-LTRVTAILTALFFVTSLTLAVFAKKQTTEAYSLK----------TVQTTAPAQTTSPETSPNAPKTGQ 4JFZ Chain:H ((45-138)) ------------------------GKGLEWVASIATGGHTTYYSDSVKGRFTISRDNSKNTLYLQMNSLRAEDTAVYYCTRGYSSTSYAMDYWGQGTLVTVSSASTKGPSVFPLAPSS--

General information: TITO was launched using: RESULT: Template: 4JFZ.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain H - contact count / total energy / energy per contact / energy per residue : 232 -4701 -20.26 -56.63 target 2D structure prediction score : 0.31 Monomeric hydrophicity matching model chain H : 0.61 3D Compatibility (PKB) : -20.26 2D Compatibility (Sec. Struct. Predict.) : 0.31 1D Compatibility (Hydrophobicity) : 0.61 QMean score : 0.175

(partial model without unconserved sides chains): PDB file : Tito_4JFZ.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4JFZ-query.scw PDB file : Tito_Scwrl_4JFZ.pdb: