Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MAKKIDGYIKLQVPAGKANPSPPIGPALGQRGVNIMAFCKEFNAATQKVEPGLPIPVVITVYNDKSFTFIMKTPPASILLKKAAGIQKGSSVPNKTKVGKLTRAQLEEIATTKEPDLTGADLDARVRTIAGSARSMGLEVEL 3J0D Chain:G ((1-140)) -AKKVQAYVKLQVAAGMANPSPPVGPALGQQGVNIMEFCKAFNAKTDSIEKGLPIPVVITVYADRSFTFVTKTPPAAVLLKKAAGIKSGSGKPNKDKVGKISRAQLQEIAQTKAADMTGADIEAMTRSIEGTARSMGLVVE-

General information: TITO was launched using: RESULT: Template: 3J0D.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain G - contact count / total energy / energy per contact / energy per residue : 592 -66172 -111.78 -472.66 target 2D structure prediction score : 0.78 Monomeric hydrophicity matching model chain G : 0.91 3D Compatibility (PKB) : -111.78 2D Compatibility (Sec. Struct. Predict.) : 0.78 1D Compatibility (Hydrophobicity) : 0.91 QMean score : 0.689

(partial model without unconserved sides chains): PDB file : Tito_3J0D.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3J0D-query.scw PDB file : Tito_Scwrl_3J0D.pdb: