Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MEQKKSTQKRNQLLAAALDVFSLYGFSGASLDEIAQLADMHKSNIFYYYENKESLYVEVLTTVLQKWLAPLQTLEA----ELEPAEALSHYLIQKIELSRSQPKASRLFALEIIQGAPHILPILKGPLKKLFKRKAKVIQTWQEQGKISPDIDPELLILNIWGLTQNYADFATQMEMVTGKTLRNRSMFQRSIEHTVHMMLYGVLPR 3DEW Chain:A ((10-148)) ---------RSRLMEVATELFAQKGFYGVSIRELAQAAGASISMISYHFGGKEGLYA----AVLQEQFACFGQLDDIRGQAGDPLAVMTAYLRWTIQRHRNNPQLLRFYTSELTNPTPCFAAIVSPAIASVIRLLAESIEAGMTRGLFRRDL-------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 3DEW.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 462 -68863 -149.05 -510.09 target 2D structure prediction score : 0.69 Monomeric hydrophicity matching model chain A : 0.67 3D Compatibility (PKB) : -149.05 2D Compatibility (Sec. Struct. Predict.) : 0.69 1D Compatibility (Hydrophobicity) : 0.67 QMean score : 0.595

(partial model without unconserved sides chains): PDB file : Tito_3DEW.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3DEW-query.scw PDB file : Tito_Scwrl_3DEW.pdb: