Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTTRIYVRASTKDQDAE---RALADLKNFALSIKGDFNEYIENESGTKLDRPVLNQLLDDSSNGDTLLVESVDRLSRLSQNDFEVLKGRIKDKGLKLVVADLPTTHMLVNSADNITSSILSLVNNMLIDLLATMARLDNDKRRERIKQGLERSGYKPT--GKKADTAKHTRIKELNNKGLTKEEIAKAVGCGVATVYRVIKK 1GDT Chain:A ((6-179)) -----YARVSTSQQSLDIQVRALKDAGVKANRI------FTDKASGSSSDRKGLDLLRMKVEEGDVILVKKLDRLGRDTADMIQLIK-EFDAQGVSIRFID-----------DGISTD--GEMGKMVVTILSAVAQAERQRILERTNEGRQEAMAKGVVFGRKRKIDRDA-VLNMWQQGLGASHISKTMNIARSTVYKVI--

General information: TITO was launched using: RESULT: Template: 1GDT.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 519 14774 28.47 87.42 target 2D structure prediction score : 0.62 Monomeric hydrophicity matching model chain A : 0.74 3D Compatibility (PKB) : 28.47 2D Compatibility (Sec. Struct. Predict.) : 0.62 1D Compatibility (Hydrophobicity) : 0.74 QMean score : 0.455

(partial model without unconserved sides chains): PDB file : Tito_1GDT.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1GDT-query.scw PDB file : Tito_Scwrl_1GDT.pdb: