Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MHLNSSIDKKMTTLNILFKTTSLCLAMALLVGCNDNDDNDSSTPAPRSNQTVNILAFNDFHGNLEPPKRYVEAANPNDASQTVRIPVGGVSYFADAIKKLKAQNPNNVVVSAGDLISASPLTSSLFLDEPTIEVMNDIQIDFNAVGNHEFDRGTDELRRLQNGGCQQYTSTAPCQINKNFSGAKFNFLAANVAMKNDTSKTI---FPAYKVKTYGGIPVAFIGLTLKATPSIVSAAGIKDVEFRDEADTVNALIPELQKQGIEAIVVVVHEGAAPSTKLNQKTCDGLSGPILG---ILDRLNPAVDIVVSGHTHQSYICDYATKNPAKPFLLTSAGQYGTLITDIKVGLDGKTGDIIKKDAKQIPVQSEAYTSGTTTVSLTDLYQKFSKTPSIEAILDKYRQAVTTISGRVVGTSTAVV--SRTQVESGESPLGDMIADAQQAAALQASNQ----GSDFTLMNPGGVRADLLINSSNQITFGDIFAVQPFGNSIVTLSLTGKQIRDVLEQQWSGANANSPRILQPSKELSYQYAANTSVSPRASNIMVAGS--------PLVDTKVYRVTVNSFLADGGDNFTVLKEGQNRV-GGGQDIDALESYVAQNSKDSPLIIPATT-RIKVIK 4H1Y Chain:P ((23-544)) ---------------------------------------------P---WELTILHTNDVHSRLEQTSE--DSSKCVDASRCM----GGVARLFTKVQQIRRAEPNVLLLDAGDQYQGT-IWFTVYKGAEVAHFMNALRYDAMALGNHEFDNGVEGL------------------IEPLLKEAKFPILSANISASGPLASQISGLYLPYKVLPVGDEVVGIVGYTSKETP-FLSNPGTNLV-FEDEITALQPEVDKLKTLNVNKIIALGHSGFEMDKLIAQKVRGVDVVVGGHSNTFLYTGNPPSKEVPAGKYPFIVTSDDGRKVP-----VVQAYAFGKYLGYLKIEFDER-GNVISSHGNPILLDS-----------------SIPEDPSIKADINKWRIKLDDYSTQELGKTIVYLDGSSQSCRFRECNMGNLICDAMINNNLRHADEMFWNHVSMCILNGGGIRSPIDERNDGTITWENLAAVLPFGGTFDLVQLKGSTLKKAFEHSVHRYGQSTGEFLQVGG-IHVVYDLSRKPGDRVVKLDVLCTSCRVPSYDPLKMDEVYKVILPNFLANGGDGFQMIKDELLRHDSGDQDINVVSTYISKMK----VIYPAVEGRIK---

General information: TITO was launched using: RESULT: Template: 4H1Y.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain P - contact count / total energy / energy per contact / energy per residue : 3012 46727 15.51 93.45 target 2D structure prediction score : 0.69 Monomeric hydrophicity matching model chain P : 0.72 3D Compatibility (PKB) : 15.51 2D Compatibility (Sec. Struct. Predict.) : 0.69 1D Compatibility (Hydrophobicity) : 0.72 QMean score : 0.374

(partial model without unconserved sides chains): PDB file : Tito_4H1Y.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4H1Y-query.scw PDB file : Tito_Scwrl_4H1Y.pdb: